>> New DNA Ligases and Ligase Master Mixes
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New DNA Ligases and Ligase Master Mixes
New England Biolabs offers the most extensive
selection of high-quality and performance optimized
DNA ligases and ligase master mixes of any supplier
to streamline your cloning experiments.
| New DNA ligase products include: |
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Click here to try a free
sample of our Blunt/TA
and Instant Sticky-end
Ligase Master Mixes, give
us your feedback and be
entered to win an iPad®2.
Offer valid while supplies last. Limit
one sample per customer. Winner will
be notified by November 30, 2012. |
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Blunt/TA Ligase Master Mix outperforms the competition.
Duplicate ligation reactions of blunt or T/A vector/insert pairs were set up according to the master mix vendors' suggestions. Equal amounts of ligated DNA were used to transform NEB 10- beta Competent E. coli (NEB #C3019) and triplicate plating was performed. Transformation results were averaged and graphed as a percentage of the highest performing reaction, T/A ligation using the Blunt/TA Ligase Master Mix.
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Q5™ AND Q5 HOT START
HIGH-FIDELITY DNA POLYMERASES |
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With ultra-high fidelity and ultra-low error rates, Q5 High-Fidelity DNA Polymerase provides superior performance with minimal optimization across a broad range of amplicons, regardless of GC content.
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| Five quality features of Q5: |
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Fidelity – the highest fidelity amplification
available (>50X higher than Taq) |
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Robustness – high specificity and yield with
minimal optimization |
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Coverage – superior performance for a broad
range of amplicons (from high AT to high GC) |
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Speed – short extension times |
| 5. |
Amplicon length – robust amplifications
up to 20 kb |
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FEATURED PRODUCTS –
PNGASE F & PNGASE F (GLYCEROL FREE) |
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- High performance – N-glycan core oligosaccharides remain intact for downstream analysis
- Convenient and flexible protocols – Fast reaction setup; use under both native and denaturing conditions; compatible with protease inhibitor cocktails
- Multiple formats – Glycerol-free format, which is compatible with downstream HPLC and mass spec analysis
- Exceptional quality – Purified to >95%; no contaminating proteases, exoglycosidases or non-specific endoglycosidases; 24-months of stability
High purity – no detectable endoglycosidase F1 activity in NEB's PNGase F
 Panel A: 20 pmol Man5-2AA. Elution off Amide-80 column occurs at 17.90 minutes with a fluorescence peak area of 13142. Panel B: 20 pmol Man5-2AA after incubation with 5,000 units of PNGase F for 20 hours at 37°C. Elution off Amide-80 column occurs at 17.77 minutes with a fluorescence peak area of 13265. Man5-2AA remains intact following PNGase F incubation, confirming no detection of Endoglycosidase F1 activity.
Order Information
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Did you know that NEB does not charge any dry ice fees for shipment of competent cells?
 Yes. |
| No, but I knew NEB supplied competent cells. |
| No, and I didn't know NEB supplied competent cells. |
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Q: |
Is PNGase F compatible with downstream analysis, such as HPLC and Mass Spectrometry, and can it be used in a double digest with O-Glycosidase? |
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Yes, PNGase F and O-Glycosidase (NEB #P0733) can be used together in a double digest under either denaturing or non-denaturing (native) conditions. Both endoglycosidases can be used concomitantly in a reaction with 1X Glycoprotein Denaturing Buffer, 1X G7 Reaction Buffer, 10% NP40, Neuraminidase (NEB #P0720), H20 and the glycoprotein of interest.
PNGase F (Glycerol Free) used under non-denaturing conditions is recommended when downstream analysis will include HPLC and/or Mass Spectrometry, due to the fact that glycerol, SDS, and NP-40 are not tolerated in those instruments.
A typical non-denaturing reaction protocol for a double digest is as follows:
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Combine 1-20 µg of glycoprotein, 2 µl of 10X G7 Reaction Buffer, 2 µl of Neuraminidase and H2O (if necessary) to make a 20 µl total reaction volume. |
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Add 5 µl of PNGase F (Glycerol Free) and 5 µl O-Glycosidase. |
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Incubate reaction at 37°C for 4 hours. |
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NEW ENGLAND BIOLABS® is a registered trademark of New England Biolabs, Inc.
ELECTROLIGASE™ and Q5™ are a trademarks of New England Biolabs, Inc.
IPAD® is a registered trademark of Apple, Inc.
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New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938-2723 |
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